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Journal: mSystems
Article Title: Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in Chlamydia trachomatis persistence
doi: 10.1128/msystems.00717-24
Figure Lengend Snippet: Overall process of the model reconstruction, refinement, and subsequent analysis/validation. Kbase, NCBI RefSeq, and ModelSEED database were used to reconstruct the initial model. After standard model curations, a high-quality GSM was ready to use. Later, condition-specific transcriptomics data were incorporated with GSM through E-flux algorithm, which resulted in five contextualized models. These models were used to find metabolic bottlenecks and persistence mechanism. K-mean clustering algorithm applied on the transcriptomics data revealed a global stress response mechanism. Thermodynamics and enzyme cost analysis, along with in vitro experimentation, reveal the regulatory role of pgm in CTL persistence.
Article Snippet: To analyze the metabolism of CTL in different stress conditions, we reconstructed a GSM of CTL using the
Techniques: Biomarker Discovery, In Vitro